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Literature summary extracted from

  • Vigetti, D.; Genasetti, A.; Karousou, E.; Viola, M.; Clerici, M.; Bartolini, B.; Moretto, P.; De Luca, G.; Hascall, V.C.; Passi, A.
    Modulation of hyaluronan synthase activity in cellular membrane fractions (2009), J. Biol. Chem., 284, 30684-30694.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.212 interleukin 1beta activation of enzyme in plasma membrane fraction Homo sapiens
2.4.1.212 phorbol 12-myristate 13-acetate activation of enzyme in plasma membrane fraction Homo sapiens
2.4.1.212 platelet-derived growth factor BB activation of enzyme in plasma membrane fraction Homo sapiens
2.4.1.212 tunicamycin significant increase in HAS activity in the cytosolic membrane fraction after tunicamycin treatment Homo sapiens

Application

EC Number Application Comment Organism
2.4.1.212 analysis quantification of newly synthesized hyaluronan by polyacrylamide gel electrophoresis of fluorophore-labeled saccharides and high performance liquid chromatography. The method measures HAS activity in the plasma membrane fraction and also in the cytosolic membranes. The technique is used to evaluate the effects of 4-methylumbeliferone, phorbol 12-myristate 13-acetate, interleukin 1, platelet-derived growth factor BB, and tunicamycin on HAS activities Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.212 additional information not inhibitory: 4-methylumbeliferone Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 membrane fraction containing membrane proteins from the endoplasmic reticulum and Golgi apparatus Homo sapiens 16020
-
2.4.1.212 plasma membrane
-
 Homo sapiens 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.212 glycoprotein HAS activity can be modulated by post-translational modification, such as phosphorylation and N-glycosylation Homo sapiens
2.4.1.212 phosphoprotein HAS activity can be modulated by post-translational modification, such as phosphorylation and N-glycosylation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 ECV-304 cell
-
 Homo sapiens
-
2.4.1.212 U-937 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 UDP-D-glucosamine + UDP-D-glucuronate
-
 Homo sapiens [beta-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + n UDP
-
 ?